CBSE Class 12 Biotechnology Syllabus 2021-22 (New): CBSE Academic Session 2021-22
(*12*)CBSE Class 12 Syllabus 2021-22 (New) Launched For CBSE Academic Session 2021-2022: Obtain Topic-wise PDF!
(*12*)CBSE Class 12 Biotechnology Syllabus 2021-22 (New):
(*12*)Unit-V Protein and Gene Manipulation
(*12*)Chapter-1: Recombinant DNA Know-how
(*12*)Introduction, Software of DNA know-how, Making DNA, Introduction of recombinant DNA into host cells, Identification of recombinants, Polymerase Chain Response (PCR), Hybridization strategies, DNA library, DNA Sequencing, Website-directed Mutagenesis
(*12*)Chapter-2: Protein Construction and Engineering
(*12*)Introduction to the world of proteins, 3-D form of proteins, Construction-function Relationship in proteins, Purification of proteins, Characterization of proteins, Protein primarily based merchandise, Designing proteins (Protein Engineering)
(*12*)Chapter-3: Genomics, Proteomics and Bioinformatics
(*12*)Introduction, Genome, Sequencing tasks, Gene prediction and counting, Genome similarity, SNPs and Comparative genomics, Useful genomics, Proteomics, Historical past of bioinformatics, Sequences and nomenclature, Info sources, Evaluation utilizing bioinformatics instruments
(*12*)Unit-VI Cell Tradition and Genetic Manipulation
(*12*)Chapter-1: Microbial Cell Tradition and its Functions
(*12*)Introduction, Microbial vitamin and tradition strategies, Measurement and kinetics of microbial progress, Scale-up of microbial course of, Isolation of microbial merchandise, Pressure isolation and enchancment, Functions of microbial tradition know-how, Biosafety points in microbial know-how
(*12*)Chapter -2: Plant Cell Tradition and Functions
(*12*)Introduction, Cell and tissue tradition strategies, Functions of cell and tissue tradition, Gene switch Strategies in vegetation, Transgenic vegetation with helpful traits, Biosafety of transgenic plant
(*12*)Chapter-3: Animal Cell Tradition and Functions
(*12*)Introduction, Animal cell tradition strategies, Characterization of cell strains, Strategies of gene supply into cells, Scale-up of animal tradition course of, Functions of animal cell tradition, Stem cell know-how, Tissue engineering
(*12*)Sensible: 30 Marks
(*12*)Notice: Each pupil will be required to do the following experiments throughout the educational session.
(*12*)1. Use of particular gear in biotechnology experiments
(*12*)2. Isolation of bacterial plasmid DNA
(*12*)3. Detection of DNA by gel electrophoresis
(*12*)4. Isolation of genomic DNA (CTAB methodology)
(*12*)5. Estimation of DNA by UV spectroscopy
(*12*)6. Bacterial transformation utilizing any plasmid
(*12*)7. Restriction digestion of plasmid DNA & its evaluation by gel electrophoresis
(*12*)8. Isolation of micro organism from curd & staining of micro organism
(*12*)9. Cell viability assay utilizing Evan’s blue dye exclusion methodology
(*12*)10. Information retrieval and database search utilizing web web site NCBI and obtain a DNA
(*12*)and protein sequence from web, analyze it and touch upon it
(*12*)11. Studying of a DNA sequencing gel to reach on the sequence
(*12*)12. Undertaking work
(*12*)Scheme of Analysis
(*12*)Time: 3 Hours, Max. Marks 30
(*12*)The scheme of analysis on the finish of the session shall be as below:
(*12*)Notice:- Extra emphasis ought to be given on palms on work in tasks.
(*12*)– A TextBook of Biotechnology – Class XII : Printed by CBSE, New Delhi
(*12*)– A Laboratory Guide of Biotechnology – Class XII : Printed by CBSE, New Delhi
(*12*)Time : 3 hrs., Most Marks: 70 Marks
(*12*)· Typology of questions: VSA together with MCQs, Assertion – Reasoning kind questions; SA; LA-I; LA-II; Supply-based/ Case-based/ Passage-based/ Built-in evaluation questions.
(*12*)· An inner alternative of roughly 33% could be offered.
(*12*)Suggestive verbs for varied competencies
(*12*)· Display, Data and Understanding
(*12*)State, title, listing, determine, outline, counsel, describe, define, summarize, and so on.
(*12*)· Utility of Data/Ideas
(*12*)Calculate, illustrate, present, adapt, clarify, distinguish, and so on.
(*12*)· Analyze, Consider and Create
(*12*)Interpret, analyse, evaluate, distinction, study, consider, talk about, assemble, and so on.
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