CBSE Class 12 Biotechnology Syllabus 2021-22 (New): CBSE Academic Session 2021-22

CBSE Class 12 Biotechnology Syllabus 2021-22 (New): CBSE Academic Session 2021-22
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CBSE Class 12 Biotechnology Syllabus 2021-22 (New): CBSE Academic Session 2021-22

CBSE Class 12 Biotechnology Syllabus 2021-22 (New): CBSE Academic Session 2021-22

CBSE Class 12 Biotechnology Syllabus 2021 22

(*12*)Examine CBSE Syllabus 2021-22 for Class 12 Biotechnology. It’s relevant for CBSE Academic Session 2021-22. Obtain hyperlink is given on the finish of this text. Obtain now and put together for CBSE Class 12 Biotechnology board examination.

(*12*)CBSE Class 12 Syllabus 2021-22 (New) Launched For CBSE Academic Session 2021-2022: Obtain Topic-wise PDF!

(*12*)CBSE Class 12 Biotechnology Syllabus 2021-22 (New): 




No. of


Unit V

Protein and Gene Manipulation



Unit VI

Cell Tradition and Genetic Manipulation












(*12*)Unit-V Protein and Gene Manipulation

(*12*)Chapter-1: Recombinant DNA Know-how

(*12*)Introduction, Software of DNA know-how, Making DNA, Introduction of recombinant DNA into host cells, Identification of recombinants, Polymerase Chain Response (PCR), Hybridization strategies, DNA library, DNA Sequencing, Website-directed Mutagenesis  

(*12*)Chapter-2: Protein Construction and Engineering

(*12*)Introduction to the world of proteins, 3-D form of proteins, Construction-function Relationship in proteins, Purification of proteins, Characterization of proteins, Protein primarily based merchandise, Designing proteins (Protein Engineering) 

(*12*)Chapter-3: Genomics, Proteomics and Bioinformatics 

(*12*)Introduction, Genome, Sequencing tasks, Gene prediction and counting, Genome similarity, SNPs and Comparative genomics, Useful genomics, Proteomics, Historical past of bioinformatics, Sequences and nomenclature, Info sources, Evaluation utilizing bioinformatics instruments 

(*12*)Unit-VI Cell Tradition and Genetic Manipulation  

(*12*)Chapter-1: Microbial Cell Tradition and its Functions 

(*12*)Introduction,  Microbial  vitamin  and  tradition  strategies,  Measurement  and  kinetics  of microbial progress, Scale-up of microbial course of, Isolation of microbial merchandise, Pressure isolation and enchancment, Functions of microbial tradition know-how, Biosafety points in microbial know-how 

(*12*)Chapter -2: Plant Cell Tradition and Functions

(*12*)Introduction, Cell and tissue tradition strategies, Functions of cell and tissue tradition, Gene switch Strategies in vegetation, Transgenic vegetation with helpful traits, Biosafety of transgenic plant 

(*12*)Chapter-3: Animal Cell Tradition and Functions 

(*12*)Introduction, Animal cell tradition strategies, Characterization of cell strains, Strategies of gene supply into cells, Scale-up of animal tradition course of, Functions of animal cell tradition, Stem cell know-how, Tissue engineering 


(*12*)Sensible: 30 Marks 

(*12*)Notice:  Each  pupil  will  be  required  to  do  the  following  experiments  throughout  the educational session. 

(*12*)1.  Use of particular gear in biotechnology experiments

(*12*)2.  Isolation of bacterial plasmid DNA

(*12*)3.  Detection of DNA by gel electrophoresis

(*12*)4.  Isolation of genomic DNA (CTAB methodology)

(*12*)5.  Estimation of DNA by UV spectroscopy

(*12*)6.  Bacterial transformation utilizing any plasmid

(*12*)7.  Restriction digestion of plasmid DNA & its evaluation by gel electrophoresis

(*12*)8.  Isolation of micro organism from curd & staining of micro organism

(*12*)9.  Cell viability assay utilizing Evan’s blue dye exclusion methodology

(*12*)10.  Information retrieval and database search utilizing web web site NCBI and obtain a DNA

(*12*)and protein sequence from web, analyze it and touch upon it

(*12*)11.  Studying of a DNA sequencing gel to reach on the sequence

(*12*)12.  Undertaking work 

(*12*)Scheme of Analysis

(*12*)Time: 3 Hours,  Max. Marks 30

(*12*)The scheme of analysis on the finish of the session shall be as below: 


Two experiments

6+6 (just one pc primarily based sensible)


Sensible report



Viva on Sensible



Undertaking work



Write up



Viva on mission





(*12*)Notice:- Extra emphasis ought to be given on palms on work in tasks. 

(*12*)Prescribed Books: 

(*12*)– A TextBook of Biotechnology – Class XII : Printed by CBSE, New Delhi

(*12*)– A Laboratory Guide of Biotechnology – Class XII : Printed by CBSE, New Delhi 


(*12*)Evaluation Areas

(*12*)(Idea) 2021-22

(*12*)Class XII

(*12*)Time : 3 hrs., Most Marks: 70 Marks 




Display Data and Understanding




Utility of Data / Ideas




Analyse, Consider and Create




(*12*)· Typology of questions: VSA together with MCQs, Assertion – Reasoning kind questions; SA; LA-I; LA-II; Supply-based/ Case-based/ Passage-based/ Built-in evaluation questions. 

(*12*)· An inner alternative of roughly 33% could be offered.

(*12*)Suggestive verbs for varied competencies

(*12*)· Display, Data and Understanding

(*12*)State, title, listing, determine, outline, counsel, describe, define, summarize, and so on.

(*12*)· Utility of Data/Ideas

(*12*)Calculate, illustrate, present, adapt, clarify, distinguish, and so on.

(*12*)· Analyze, Consider and Create

(*12*)Interpret, analyse, evaluate, distinction, study, consider, talk about, assemble, and so on.

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